Beyond Gene Delivery: Strategies to Engineer the Surfaces of Viral Vectors
نویسندگان
چکیده
منابع مشابه
Beyond Gene Delivery: Strategies to Engineer the Surfaces of Viral Vectors
Viral vectors have been extensively studied due to their great transduction efficiency compared to non-viral vectors. These vectors have been used extensively in gene therapy, enabling the comprehension of, not only the advantages of these vectors, but also the limitations, such as the activation of the immune system after vector administration. Moreover, the need to control the target of the v...
متن کاملStrategies to test nuclear localization of non-viral gene delivery vectors in vitro and in vivo
Non-viral gene delivery is plagued by low transfection levels compared to viral delivery. The nuclear envelope presents a significant obstacle for non-viral vectors. A peptide-based nuclear localizing sequence has been incorporated into non-viral vectors to traverse the nuclear envelope. Here, we selected a photo-chemical method for covalently labeling the peptide onto plasmid DNA. The hypothes...
متن کامل"Bronchial Artery Delivery of Viral Vectors for Gene delivery in Cystic Fibrosis; Superior to Airway Delivery?"
BACKGROUND Attempts at gene therapy for the pulmonary manifestations of Cystic Fibrosis have relied mainly on airway delivery. However the efficiency of gene transfer and expression in the airway epithelia has not reached therapeutic levels. Access to epithelial cells is not homogenous for a number of reasons and the submucosal glands cannot be reached via the airways. PRESENTATION We propose...
متن کاملGene Delivery into the Brain Using Viral Vectors
The delivery of recombinant genes into the brain is becoming an increasingly important strategy for answering questions about the molecular mechanisms of brain function. Answers to these questions may be applied to many of the disorders that affect the brain. For example, an understanding of the mechanisms by which repeated exposure to drugs of abuse increases their stimulant and rewarding prop...
متن کاملViral vectors for gene delivery in tissue engineering.
The goal of tissue engineering is the production of functional, biocompatible tissues by seeding cells within biological or synthetic scaffolds. One tissue engineering approach involves the genetic modification of cells that are seeded onto (or into) scaffolds prior to implantation. The genetic modification is achieved through gene delivery, with can utilize viral transduction or non-viral tran...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biomedicines
سال: 2013
ISSN: 2227-9059
DOI: 10.3390/biomedicines1010003